Nutraceutics

BRING PROOF OF EFFICACY
Biological evaluations for health ingredients to valorize an immuno-modulatory potential,
prevent diseases and maintain an healthy balance.

Expertise of Ambiotis : Innovative approaches for the valorization of nutritional products

Ambiotis is a research-based company specialized in the understanding of inflammatory processes, immunity and well-being associated with an healthy body balance.

Ambiotis develops validated models and technologies to bring proofs of concept of ingredients and help its business partners put faster on the market innovative and potent nutritional solutions.

Ambiotis has the ability to set up full research programs from screening assays up to monitoring of biomarkers in clinical trials.

How to evaluate nutritional ingredients ?

Inflammation is a natural response of our immune system to fight injury or infection. If correctly controlled, it leads to the return to a healthy balance.

This return to basal conditions is called resolution and is characterized by the natural and sequential synthesis of specialized pro-resolving mediators (resolvins, protectins, lipoxins, maresins) or SPMs. These mediators are essential to induce correct healing and prevent from chronic inflammation.

SPMs are issued from the metabolism of omega-3 and omega-6 polyunsaturated fatty acids (PUFAs): ARA, DHA, EPA and DPA.

The conversion of these PUFAs to SPMs has been linked to the well-known disease preventing- and health promoting-effects of DHA and EPA.

Discovery of these mediators and their role has thus brought new prospects for the development of ingredients notably in the field of nutrition.

Companies are thus starting to develop products that contain SPMs or that have the capacity to induce the production of these beneficial mediators.

These approaches represent a promising strategy to help the body resolving inflammatory and immune challenges without immuno-suppressive drawbacks.

Ambiotis has developed a step-by- step approach on immuno-modulation and/or pro- resolution strategies. 

Any step can also be performed on its own.

  • In vitro screening of ingredient potential (secretory and functional approaches in human primary cells)
  • In vivo proof-of- concept. Use of very well characterized and reference models in the field. Our models allow the understanding of the physiological response to a product and quantification of beneficial effects thanks to the calculation of resolution indices....
  • In disease models and/or human monitoring of resolution mediators

From screening to physiology

Raw materials analysis

- Active substance monitoring (lipids, …)

Bioavailability validation

- Monitoring in animals of product availability in blood or tissues

In vitro and in vivo efficacy screening

- Immuno-stimulating/immuno-modulating properties

- Anti-inflammatory properties

- Pro-resolutive properties

Efficacy validation

  • Animal models in numerous health areas

- comparison to a reference molecule

- physiological, clinical and blood parameters

- tissue and cell analysis (histology and cytometry)

- cell function (mediator secretion …)

- gene expression

  • Clinical trials in humans: monitoring of mediators into the blood or urine

Functional solutions

Immune system equilibrium and boost

- Whole blood and PBMCs (cytokines and lipid secretion…)

- Elimination of infectious agents by monocyte/macrophages (phagocytosis)

- Defence capacities (ROS production…)

- Immune cell differentiation (dendritic cells maturation…)

- Immune cell recruitment to inflamed/infected site in mice

- Infectious agents clearance in mice

Obesity and weight management

- Pre-adipocytes and adipocytes

- Immune cells (macrophages…)

- HFD induced obesity in mice and rats

- Ob/Ob and Db/Db mice

Respiratory troubles

- Smoke-exposed cells

- Alveolar macrophages extracted from mice

- Cigarette smoke-exposed mouse model

- LPS lung challenge in mice

Allergic troubles

- Granulocytes degranulation (histamine release…) in cell culture

- Ovalbumin-induced allergy in mice

- House Dust Mite-induced allergy in mice

Gut health

- Immune cells (monocytes, macrophages, neutrophils…)

- DSS-induced colitis in mice

- TNBS-induced colitis in mice

- Ischemia/reperfusion in mice

Bone and Joint Health

- Immune cells (monocytes, macrophages, neutrophils…)

- Collagen-induced arthritis

- Carrageenan-induced paw edema

- Air pouch

- mBSA-induced arthritis

Skin Health and Nutricosmetics

- Pre-adipocytes and adipocytes

- Keratinocytes

- Fibroblasts

- Melanocytes

- Immune cells (Langerhans cells, lymphocytes)

- Co-culture

- Reconstructed human skin

- Skin explant

Analytical services

Lipid analysis

- Eicosanoids (Inflammatory mediators, SPMs)

- Endocannabinoids

- Fatty acids: DHA, EPA, ARA, omega-3 index, sapienic acid, sebaleic acid…

- Cholesterol, sterols, triglycerides

- Squalene

Techniques: LC-MS/MS or GC-FID

Cytokine and chemokines

- Inflammatory cytokines and chemokines

- Anti-inflammatory cytokines

Techniques: ELISA, Milliplex, Flow cytometry

Gene expression

- Single gene expression

- Profiling using microfluidic dynamic array

Techniques: RT-qPCR

Oxidative stress markers

- Reactive oxygen species

Techniques: Luminometry (real-time assays), Flow cytometry (accumulation), Fluorometry, Mass spectrometry

Cells and Tissues

Techniques : Flow cytometry, Histology

Ways of working with us

 

Step 1: your need

  • Understanding of your need
  • Orientation toward the relevant work package

- analytical package : provide us with samples to be analyzed

- model-based package : provide us with your compounds to evaluate

- Customized development

- Scientific consulting

Step 2: our collaboration

  • Performance of services according milestones and go/no go steps
  • Regular follow-up

Step 3: your results

  • Scientific feedback through webex conference
  • Final report
  • Follow up