Ambiotis proposes fully validated experimental protocols. A custom-made strategy can also be elaborated thanks to a technology block assembly, in order to turn research issues into experimental solutions.
Models
Our cell-based assays (human primary cells) and in vivo models (mice and rats) allow to address multiple research questions:
- Inflammation
- Resolution
- Tissue and skin protection
- Immuno-modulation
- Immuno-toxicity
- Senescence
- Cytotoxicity
- Cell function (phagocytosis, oxidative burst…)
- Compound bioavailability
- Other : please contact us
analytical Technologies
Cytometry
- Identification and characterization of cell populations or receptors
- Reactive oxygen species quantification
- Intracellular cytokines or chemokines
Fluorometry and luminometry
- Macrophage phagocytosis capacities
- Reactive oxygen species quantification
- Quantification of cytokines, chemokines or lipids (ELISA/EIA)
Real-Time PCR
- Gene expression profiling
- Nanofluidic array
Multiplex technology
- Quantification of cytokines or chemokines
Lipid quantification (LC-MS/MS ou GC-FID)
- Eicosanoids (Inflammatory mediators, SPMs)
- Endocannabinoids
- Fatty acids (saturated, mono/poly-unsaturated…) : omega-3, omega-6, skin fatty acids…
- Cholesterol, sterols, triglycerides
- Squalene
Histology
- Inflammatory cell infiltration
- Tissue integrity
- Disease scoring